What Gram stain is Staphylococcus epidermidis?
Staphylococcus epidermidis is a coagulase-negative, gram-positive cocci bacteria that form clusters. It is also a catalase-positive and facultative anaerobe.
What color does Staphylococcus epidermidis stain?
The acid-fast Mycobacterium retains carbol fuchsin and stains hot pink. The Staphylococcus epidermidis is decolorized and the counterstain colors them blue.
Why is S. epidermidis gram-positive?
S. epidermidis is a gram-positive bacterium. Its cell wall teichoic acid is formed by polymerized glycerol, glucose, and N-acetyl glucosamine.
What color should be seen when viewing a Gram stain of Staphylococcus epidermidis?
What color is Staphylococcus epidermidis under a gram stain? Why? Purple because it is gram postive. It does have a thick peptidoglycan cell wall and therefore retains the crystal violet stain.
What does S. epidermidis cause?
However, S. epidermidis can cause opportunistic infections, which include particularly biofilm-associated infections on indwelling medical devices. These often can disseminate into the bloodstream; and in fact, S. epidermidis is the most frequent cause of nosocomial sepsis.
Does S. epidermidis ferment?
Anaerobically grown Staphylococcus epidermidis fermented glucose with the production of lactate and trace amounts of acetate, formate and CO2. Glucose-grown organisms at various stages of anaerobic batch growth showed an inverse relationship between the rates of fermentation of serine and glucose.
Is Gram-positive pink or purple?
If the bacteria stays purple, they are Gram-positive. If the bacteria turns pink or red, they are Gram-negative. The two categories cause different types of infections: Gram-positive infections include methicillin-resistant Staphylococcus aureus (MRSA), strep infections, and toxic shock.
Is S. epidermidis lipase positive?
Lipase enzyme was detected in 15 (83.3%) S. aureus-positive isolates and nine (100%) S. epidermidis-positive isolates.
What does a Gram stain tell you?
What is it used for? A Gram stain is most often used to find out if you have a bacterial infection. If you do, the test will show if your infection is Gram-positive or Gram-negative. A Gram stain may also be used to diagnose fungal infections.
What is the principle of Gram staining?
The basic principle of gram staining involves the ability of the bacterial cell wall to retain the crystal violet dye during solvent treatment. Gram-positive microorganisms have higher peptidoglycan content, whereas gram-negative organisms have higher lipid content.
What are the signs and symptoms of a S. epidermidis infection?
What are symptoms of the disease? Staphylococcus epidermidis can cause wound infections, boils, sinus infections, endocarditis and other inflammations. The bacterium can reside for a long period of time in “hiding places” in the body, where it is not noticed by the immune system, and therefore also not fought.
Is S. epidermidis infectious?
Staphylococcus epidermidis is a common symbiont bacterium that can become infectious once inside the human host. They are among the most common causes of nosocomial infection in the United States and can lead to serious complications.
What causes Staphylococcus epidermidis?
Most, but not all, human bacterial infections are caused by coagulase-positive Staphylococcus aureus strains. Staphylococcus epidermidis strains and other Staphylococcus species that are coagulase-negative produce slime that interferes with immune defenses.
What is the Gram stain procedure?
Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet.
What is the morphology of Gram staining?
• Gram stain reaction: Gram-negative diplococci. • Morphology: medium, smooth round, moist, gray to white, encapsulated strains are mucoid, may be greenish cast in agar underneath colonies.
Does Staphylococcus epidermidis have motility?
Staphylococcus epidermidis | Microbiology. The SIM tube also tests for sulfur reduction and motility. The unknown was positive for Indole production, motility, and sulfur reduction. These results were indicated by a red color change at the top of the tube that indicated Indole production, black coloring that indicated sulfur reduction,…