What does the transformation experiment tell us about bacteria?
Griffith’s experiment, reported in 1928 by Frederick Griffith, was the first experiment suggesting that bacteria are capable of transferring genetic information through a process known as transformation.
What happens during a transformation experiment?
Transformation is a key step in DNA cloning. It occurs after restriction digest and ligation and transfers newly made plasmids to bacteria. After transformation, bacteria are selected on antibiotic plates. Bacteria with a plasmid are antibiotic-resistant, and each one will form a colony.
What are the two genes on the pBLU plasmid?
pBLU is a commercially produced bacterial plasmid that contains genes for ampicillin resistance (beta lactamase and beta galactosidase).
What is the purpose of the transformation lab?
A laboratory activity that allows students to investigate some basic principles of molecular biology and an introduction to genetic engineering. Plasmids containing the pFluoroGreen gene and the ampicillin resistance gene will be used to transform E. coli bacteria cells.
What did the Griffith experiment prove?
In his famous experiments, Griffith [66] demonstrated in 1928 that the ability to synthesis a capsule and hence become virulent can be transferred from heat-inactivated “smooth” strains to living but avirulent “rough” strains of S. pneumoniae.
How can you tell if a transformation experiment has been successful?
How can you tell if a transformation experiment has been successful? If transformation is successful, the DNA will be integrated into one of the cell’s chromosomes. How are genetic markers related to transformation?
Why is there no growth on LB amp plate?
What is the purpose of the –plasmid plates? The LB/AMP -plasmid plate should show no growth because the bacteria have not been exposed to the plasmid with ampicillin resistance.
Which plate is the positive control in our transformation experiment?
growth plate
In a bacterial transformation experiment, growing untransformed bacteria on a regular growth plate is considered a positive control with respect to growth because we expect the bacterial cells to grow.
How did Frederick Griffith prove that some transforming principle?
Griffith was able to show that if you heat kill a Type IIIS strain and injected it into the mouse, the mouse lived. These meant that some material from the Type IIIS strain was taken up by the Type IIR strain to convert it into the Type IIIS strain. Griffith termed the material the transforming principle.