What is a tissue microarray used for?
Tissue microarray is a practical and effective tool for high-throughput molecular analysis of tissues that is helping identify new diagnostic and prognostic markers and targets in human cancers.
How do you do tissue microarray?
In the tissue microarray technique, a hollow needle is used to remove tissue cores as small as 0.6 mm in diameter from regions of interest in paraffin-embedded tissues such as clinical biopsies or tumor samples. These tissue cores are then inserted in a recipient paraffin block in a precisely spaced, array pattern.
What is a tissue Arrayer?
Tissue MicroArray (TMA) extracts tissue from donor blocks and transplants them to recipient blocks according to the layout designed by the users. Integrated software allows information of the samples and the recipient block to be transfered directly using database interface.
What are tissue cores?
The Tissue Core (TC) is the central biorepository for Moffitt Cancer Center. The overall goal of the TC is to collect, process, store, and release high-quality, well-annotated biospecimens in support of research.
What does TMA stand for and what are they useful for?
Abstract. The tissue microarray (TMA) technology provides the means for high-throughput analysis of multiple tissues and cells. The technique is used within the Human Protein Atlas project for global analysis of protein expression patterns in normal human tissues, cancer and cell lines.
What is TMA core?
Tissue microarrays (TMAs) are constructed by removing a core (tube shaped section) of tissue from a paraffin block (donor block) using a hollow needle and transferring this core to a predetermined position on a paraffin block (recipient block).
What is FFPE testing?
FFPE is a form of preservation and preparation for biopsy specimens that aids in examination, experimental research, and diagnostic/drug development. A tissue sample is first preserved by fixing it in formaldehyde, also known as formalin, to preserve the proteins and vital structures within the tissue.
What are FFPE sections?
Formalin fixed, paraffin embedded (FFPE) tissue sections also known as paraffin tissue sections are an ideal way for rapidly determining the cellular localization of DNA, RNA and protein markers.
What is TMA in IHC?
Tissue Microarray (TMA) refers to the practice of relocating multiple tissue sections, often from conventional histologic paraffin blocks, so that tissues from multiple samples can be evaluated on the same slide.