What is phase lock gel?
Phase Lock Gel (PLG) is a unique product that eliminates interphase-protein contamination during phenol extraction and ensures faster results with improved recoveries. PLG migrates to form a tight seal between the phases of an aqueous/organic extraction during centrifugation.
What is the advantage of using phase lock gel tubes to purify the DNA samples?
The benefits are increased yields of up to 30%, increased protection from exposure to hazardous compounds, and no risk of interphase sample contamination.
What is the main reason for using the PLG tube?
The use of PLG can result in the recovery of 20 to 30% more nucleic acid than with traditional methods. Multiple extractions can be performed in the same tube as long as maximum sample volume is not exceeded.
Does TRIzol inactivate RNases?
Remember that TRIzol can only inactivate RNases with which it is in direct contact—therefore tissue samples are not safe from RNA degradation until completely homogenized.
How do you store Trizol?
From the Trizol protocol: “Homogenized samples can be stored at room temperature for several hours, or at –60 to –70°C for at least one month.”
What is phenol chloroform isoamyl alcohol?
Phenol:chloroform:isoamyl alcohol also known as Trizol, and various names in other DNA/RNA extraction kits, is a common molecular biology reagent for extracting nucleic acids. The mixture is approximately 50-70% phenol, 30-50% chloroform, and 1-5% isoamyl alcohol.
Is TRIzol phenol chloroform extraction?
Uses in extraction The correct name of the method is guanidinium thiocyanate-phenol-chloroform extraction. The use of TRIzol can result in DNA yields comparable to other extraction methods, and it leads to >50% bigger RNA yield.
How long can cells stay in TRIzol?
If the biological sample is efficiently lysed in TRIzol and the reagent can inactivate the nucleases, RNA can be safely stored for 3 or 4 days at room temperature (20-25ºC).
Where should TRIzol reagent be stored?
After homogenization (before addition of chloroform), samples can be stored at 4 degrees C overnight or at –70 degrees C for at least 1 year. Homogenized samples can sit at room temperature for several hours before chloroform is added.
How does TRIzol reagent work?
TRIzol® Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization. The simplicity of the TRIzol® Reagent method allows simultaneous processing of a large number of samples.