How does streptozotocin induce diabetes in mice?

How does streptozotocin induce diabetes in mice?

The multiple, low-dose STZ approach is now widely used to produce an animal model of T1DM (Wu & Huan, 2007). Using this protocol, diabetes is induced by the administration of multiple, low doses of STZ (40 mg/kg, intraperitoneally, i.p.) to mice on 5 consecutive days.

How do you induce diabetes in mice?

Diabetes is induced in mice by using streptozotocin (STZ), a compound that has a preferential toxicity toward pancreatic β cells.

What type of diabetes does streptozotocin induce?

Streptozotocin-induced diabetic murine models develop type 1 diabetes, due to the cytotoxic glucose analogue streptozotocin (STZ) that is toxic to pancreatic β-cells and causes insulin deficiency. STZ methylates DNA, causing DNA fragmentation and killing pancreatic β-cells [95,97].

What are STZ mice?

Streptozotocin (STZ) is an antibiotic that produces pancreatic islet β-cell destruction and is widely used experimentally to produce a model of type 1 diabetes mellitus (T1DM). Detailed in this unit are protocols for producing STZ-induced insulin deficiency and hyperglycemia in mice and rats.

How do rats induce streptozotocin in diabetes?

For induction of experimental diabetes in male adult rats weighted 250-300 grams (75-90 days), 60mg/kg of Streptozotocin was injected intravenously. Three days after degeneration of beta cells, diabetes was induced in all animals.

What is streptozotocin injection?

STZ is a broad-spectrum antibiotic that is toxic to the insulin producing β cells of pancreatic islets. It is currently used clinically for the treatment of metastatic islet cell carcinoma of the pancreas [12] and has been used investigationally in a wide variety of large and small animal species [2–5, 13–17].

What is Hypo Insulinemia?

The ensuing hypoinsulinemia allows lipolysis and proteolysis from skeletal muscles that supply amino acids for visceral pool preservation and hepatic synthesis of glucose necessary for brain, heart, and kidney metabolism and for the oxidation of fatty acids.

How does alloxan induce diabetes in rats?

Anti-diabetic Activity in Alloxan Induced Diabetes Model Diabetes was induced in 80 male albino rats by injecting 120 mg/kg of Alloxan monohydrate intra-peritoneally dissolved in 0.9% w/v cold normal saline to overnight-fasted rats (12 h).

Is streptozotocin an antibiotic?

Streptozotocin, a new antibacterial antibiotic.

Can mice get type 2 diabetes?

The ob/ob mouse model is a monogenic model of type 2 diabetes. The animals are homozygous for a mutation in leptin and develop obesity, hyperphagia, glucose intolerance, insulin resistance and transient hyperglycemia. With age, hyperglycemia wanes in these mice due to compensating hyperinsulinemia.

What does nesidioblastosis mean?

Nesidioblastosis is defined as the proliferation of both ductular and islet cells, with hypertrophy of beta cells in islets and the formation of ductuloinsular complexes (closely associated groups of proliferating endocrine cells and small exocrine ducts).