What is a rolony?
situ. via rolling-circle amplification to create a localized “rolling circle colony” (rolony) consisting of identical copies of the parent molecule. A series of biochemical steps is then carried out.
What is slide seq?
We developed Slide-seq, a method for transferring RNA from tissue sections onto a surface covered in DNA-barcoded beads with known positions, allowing the locations of the RNA to be inferred by sequencing. The functions of complex tissues are fundamentally tied to the organization of their resident cell types.
What is seqFISH?
seqFISH (sequential Fluorescence In Situ Hybridization) is a technology that enables the identification of thousands of molecules like RNA, DNA, and proteins directly in single cells with their spatial context preserved.
What is RNA fish?
RNA FISH (RNA fluorescence in situ hybridization) is a powerful technique that enables the visualization and localization of RNA and protein targets in fixed cells. RNA FISH using bDNA technology results in greater specificity, lower background, and higher signal-to-noise ratios.
How does in situ sequencing work?
In situ sequencing (ISS) is a new method by which mRNA is sequenced directly in a section of fixed tissue or cell sample. The ability to generate mRNA expression profiles with location information within tissues is a powerful tool.
How does slide seq work?
In Slide-seq, freshly frozen tissue can be sliced onto prepared arrays of DNA-barcoded beads, causing RNA in the tissue to transfer onto the beads. Subsequent library preparation yields data that is equivalent to single cell RNA sequencing data, but with a spatial location associated with each bead.
What is spatial sequencing?
Geo-seq is a method that utilizes both laser capture microdissection and single-cell RNA sequencing procedures to determine the spatial distribution of the transcriptome in tissue areas approximately ten cells in size. The workflow involves removal and cryosectioning of tissue followed by laser capture microdissection.
What is a chromosome painting?
Chromosome ‘painting’ refers to the hybridization of fluorescently labeled chromosome-specific, composite probe pools to cytological preparations.