What is MLST used for?

What is MLST used for?

Multilocus sequence typing (MLST) is an unambiguous procedure for characterising isolates of bacterial species using the sequences of internal fragments of (usually) seven house-keeping genes.

How is MLST performed?

The principle of MLST is simple: the technique involves PCR amplification followed by DNA sequencing. In the data analysis step, all unique sequences are assigned allele numbers and combined into an allelic profile and assigned a sequence type (ST).

What is MLST analysis?

Multilocus sequence typing (MLST) is a molecular typing technique whereby a number of well chosen housekeeping genes (loci) are sequenced, usually in part. Instead, each sequence for a given locus is screened for identity with already known sequences for that locus. …

What class of genes is used in MLST Analyses?

Multilocus sequence typing (MLST) is a recently developed strain-typing system that focuses strictly on conserved housekeeping genes (5, 10, 31, 53).

What is the difference between MLST and MLSA?

MLST was first proposed in 1998 by Maiden and colleagues. Moreover, MLST is usually applied to strains that belong to a well-defined species while MLSA is more often used when species boundaries are not well known and MLSA data are used to improve species descriptions.

What is whole genome MLST?

Whole-genome MLST (wgMLST) analyses enable the recognition of genetic relationships between epidemiologically associated isolates and isolates that potentially have the same infection source.

Which of these is a useful application of multi locus sequence typing?

Multilocus sequence typing (MLST) has become a useful tool for studying the genetic diversity of important public health pathogens, such as Chlamydia trachomatis (Ct).

What is the difference between sequence type and clonal complex?

MLST involves the sequencing of seven housekeeping genes and each unique allelic profile is assigned a sequence type (ST) [3]. Clonal complexes are defined as groups of STs in which every ST shares at least five of seven identical alleles with at least one other ST in the group [4].