What is histochemical techniques?

What is histochemical techniques?

Histochemistry is an important technique that is used for the visualization of biological structures. As such, it is concerned with the identification and distribution of various chemical components of tissues through the use of stains, indicators as well as microscopy.

What is histochemistry in botany?

Histochemistry is the branch of histology dealing with the iden- tification of chemical components of cells and tissues. Fats are widely distributed in the plant body and they probably occur in small amount in every plant cell [4].

What is histochemical assay?

6.10. 3.5 Special Histochemical Assays – Quantitative Histochemistry. Histochemistry refers to procedures in which tissue sections act as the medium in which biochemical reactions are carried out by the addition of substrates, inhibitors, or other chemicals.

What is histochemical stain?

A staining method used to detect polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids, and mucins in tissues and fungal hyphae.

What is a histochemistry used for?

The Enzyme-histochemistry method is used to study the metabolic changes in the tissue of diseased patients; in the diagnostic of Hirschsprung disease; Biopsy analysis of muscle; and for the research in toxicology.

What is the principle of histochemistry?

A basic principle of histochemical methodology is the bonding of a definite chemical component of cells with a stain or the formation of a stain in the process of the reaction. A number of methods (cytophotometry and luminescence and interference microscopy) are based on the physical properties of substances.

What is the difference between histochemistry and histology?

So, whereas histology in general is the study of biological cells and tissues in fine microscopic detail using special histological techniques, histochemistry is concerned specifically with the chemicals within, between, and forming the biological cells and tissues themselves.

What is enzyme histochemical?

Enzyme Histochemistry or Histoenzymology Enzyme histochemistry is used to demonstrate the activity of enzymes present on tissues. The visualization is based on the action of the enzyme on a specific substrate. Following this reaction, an insoluble product develops providing the location of enzyme.

What are the histological techniques?

Histological staining is a commonly used medical process in pathological diagnosis and forensic studies. The process of histological staining takes five key stages, and they include fixation, processing, embedding, sectioning and staining.

How do you do histology?

The Five Steps of Histology Slide Preparation

  1. Tissue fixation. Slide preparation begins with the fixation of your tissue specimen.
  2. Specimen Transfer to Cassettes. After fixation, specimens are trimmed using a scalpel to enable them to fit into an appropriately labeled tissue cassette.
  3. Tissue Processing.
  4. Sectioning.
  5. Staining.