How do you use Sephadex LH-20?
In solvents such as chloroform, Sephadex® LH-20 is less dense than the solvent and the medium will ﬂoat. Pour the medium into the column and drain until the second adapter can be inserted. Lock the adapter in position at the surface of the medium and direct the ﬂow of chloroform upwards.
What is LH-20?
Sephadex® LH-20 is a crosslinked dextran-based resin used with organic solvent mobile phases for gel permeation, normal phase partition, and adsorption chromatography of lipids, steroids, fatty acids, hormones, vitamins, etc.
What is the MW of Sephadex?
Molecular Weight. 272.25. Component Compounds. CID 448388 (beta-D-allose) CID 753 (Glycerol)
How do you clean LH 20 columns?
After washing (if you use NaOH or HCl as suggested above, wash several times with water to remove the acid or base), let it dry, swell again in your mobile phase, and repack the column. Wash the column with mild acid, followed by distilled water and finally wash the column with desired solvent system.
How do you swell Sephadex?
How do I swell Sephadex? Sephadex is supplied as a dry powder and must be allowed to swell in excess buffer before use (see steps below). After swelling, adjust with buffer to form a thick slurry from which air bubbles are removed under vacuum. Approximately 75% settled medium is suitable.
How does a Sephadex column work?
Gel filtration based on Sephadex enables group separation of biomolecules that are above the exclusion limit of the medium, from contaminants such as salts, dyes, and radioactive labels. Sephadex is prepared by cross-linking dextran with epichlorohydrin.
How does Sephadex chromatography work?
How is Sephadex made?
Sephadex is a gel filtration resin prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range.
Is Sephadex hydrophilic?
Sephadex LH-20 is beaded, cross-linked dextran that has been hydroxypropylated to yield a chromatography medium with both hydrophilic and lipophilic character. Due to its dual character, Sephadex LH-20 swells in water and a number of organic solvents.
What is G in Sephadex g25?
Sephadex is a gel filtration resin prepared by crosslinking dextran with epichlorohydrin. Sephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-25 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine).