How do you dissolve siRNA dharmacon?
To dilute the 5x siRNA Buffer to 1x siRNA Buffer, mix four volumes of sterile RNase-free water with one volume of 5x siRNA Buffer. The composition of the 1x siRNA Buffer is 60 mM KCl, 6 mM HEPES-pH 7.5, and 0.2 mM MgCl2.
Can you resuspend siRNA in water?
SiRNA buffer is recommended for long term storage, you can resuspend your SiRNA in RNAse free molecular grade water (or 0.22µm filtered DEPC treated water) for immediate use.
What is Accell siRNA?
Guaranteed gene silencing in difficult-to-transfect cells. A novel siRNA for difficult-to-transfect cells, modified to require no transfection reagent or viral vector for delivery. Predesigned Accell siRNA is available as individual reagents and in SMARTpool format.
How do you reconstitute siRNA Sigma?
Resuspend each duplex with 100 µL of molecular biology grade water (W4502). There is no need for buffer as it is already included in the dry siRNA material. Remove 50 µL from each of the individual siRNA and transfer to a new tube. The remaining siRNA solution in each tube can be used for additional studies.
Can I Vortex siRNA?
Incubate the diluted siRNA mixture at room temperature for 5 min. Important: do NOT vortex the diluted siRNA mixture. Incubate at room temperature for 5-30 minutes (5 minutes is typically sufficient).
What does siRNA transfection do?
A siRNA transfection is the insertion of siRNA into a cell, a process that can be invaluable to gene silencing experiments. Research has shown that small interfering RNA (siRNA) is extremely valuable in silencing gene expression and enables the studying of gene functions in a multitude of cells.
Why choose sigenome Dharmacon RNAi products?
GE Dharmacon RNAi products encompass the most complete portfolio of innovative tools for transient, long-term, inducible and in vivo RNAi applications. siRNA – Pre-designed genome-wide reagents for siGENOME, specificity-enahanced ON- TARGETplus,
How are siRNA off-targets mediated by seed-region interactions?
Dharmacon scientists and collaborators demonstrated in 2006 (see Reference 1) that siRNA off-targets are mediated by antisense seed-region interactions, initiating the development of a dual-strand modification pattern: Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake
Why choose sigenome as your siRNA reagent?
Since its launch in 2004, siGENOME has provided the longest standing siRNA guarantee on the market. siGENOME designs incorporate rational strand bias with application of ON-TARGET modifications when required for optimal functionality GE Dharmacon siRNA reagents are available in the following formats:
What are non-targeting and pooled siRNA controls?
Our panel of non-targeting controls permits assessment of potential non-specific effects, to find the optimal negative control for your cell type, and your assay. Pooled siRNA controls are recommended for further reduction of off-targets, and for use with SMARTpool siRNA reagents.