What are the methods of estimating urine protein?

Several methods are available for the estimation of total proteins in urine including turbidimetric methods such as sulfosalicylic acid (SSA) [1], sulfosalicylic acid with sodium sulphate (SSSS) [2, 3], trichloroacetic acid (TCA) [4], or benzethonium chloride (BEC) [5] and the protein dye-binding methods utilizing …

Why is the biuret method not applicable in the quantitation of CSF protein?

Why is the biuret method of protein analysis not suitable for urine or CSF protein? It is not sensitive enough for the small amount of protein in the urine. Trichloracetic acid can be used to precipitate protein in urine and CSF. Hemoglobin in the serum would increase the protein level.

Which is the quantitative method of urinary protein estimation?

We assessed five quantitative methods for the determination of urinary total proteins, three of them with precipitation followed by spectrophotomollowed by spectrophotometry (Tsuchyga/Benedict, Tsuchyga/Biuret and TCA/Biuret); one with gel-filtration (Sephadex G50/Biuret) and a Turbidimetric procedure (SSA). 2.

What tests are done for protein in urine?

The name of the urine test that measures the level of albumin in your urine is called the urine albumin-to-creatinine ratio (UACR). A UACR compares the level of albumin to the level of creatinine (a waste product in your blood that comes from your muscles).

Is 3+ protein in urine normal?

While a small amount is normal, a large amount of protein in urine may indicate kidney disease.

What dye may be used for staining protein bands following electrophoresis?

Coomassie Brilliant Blue (CBB) and amido black are two dyes that are commonly used for staining the protein bands following electrophoresis [2, 3]. Generally, overnight staining followed by overnight destaining is the standard practice.

What is the indication of the intensity of the purple end color in total protein measurement?

Biuret reagent is an alkaline solution of 1% CuSO4, copper sulfate. The violet color is a positive test for the presence of protein, and the intensity of the color is proportional to the number of peptide bonds in the solution.