What is APC Petrifilm?

Aerobic Plate Count (APC)1 3M Petrifilm contains nutrients and 2,3,5-triphenyltetrazolium chloride as an indicator of bacterial growth. Reduction of triphenyltetrazolium by bacteria results in red coloured colonies2. Plates are hydrated with sample and gelling agents cause the media to solidify.

What is Petrifilm method?

The 3M™ Petrifilm™ Aerobic Count Plate is a ready-made culture medium system that contains modified Standard Methods nutrients, a cold-water-soluble gelling agent and an indicator that facilitates colony enumeration. 3M Petrifilm Aerobic Count Plates are used for the enumeration of aerobic bacteria.

How do you calculate APC?

Plates with 25-250 CFU.

1. Calculate the APC as follows: where N = Number of colonies per ml or g of product. Σ c = Sum of all colonies on all plates counted.
2. When counts of duplicate plates fall within and without the 25-250 colony range, use only those counts that fall within this range.

What are two advantages of Petrifilm plates over petri dishes?

The study found that when comparing raw materials, manufacturing, packaging, use and disposal, 3M™ Petrifilm ™ Plates used 75% less energy and 80% less water, emitted 75% less greenhouse gas and produced 66% less waste.

How do you rehydrate Petrifilm?

Allow hydrated plates to remain closed for a minimum of 1 hour before use. If using Staph Express Count Petrifilm™, hydrate plates with 1 mL of appropriate sterile diluent. Refrigerate hydrated plates for a minimum of three days before using.

What is a APC code?

APC Codes (Ambulatory Payment Classifications) APCs or Ambulatory Payment Classifications are the United States government’s method of paying for facility outpatient services for the Medicare (United States) program. APCs are an outpatient prospective payment system applicable only to hospitals.

What does TNTC mean in urinalysis?

In samples with very high bacterial concentrations, labs are often unable to get accurate counts and. report the results as “too numerous to count” (TNTC).

What is high WBC in urine?

If your doctor tests your urine and finds too many leukocytes, it could be a sign of infection. Leukocytes are white blood cells that help your body fight germs. When you have more of these than usual in your urine, it’s often a sign of a problem somewhere in your urinary tract.

Why is a Petrifilm used to culture bacteria?

They are heavily used in many microbiology-related industries and fields to culture various micro-organisms and are meant to be a more efficient method for detection and enumeration compared to conventional plating techniques. A majority of its use is for the testing of foodstuffs.

How is the 3M Petrifilm rapid aerobic count plate read?

The 3M Petrifilm Rapid Aerobic Count Plate can also be read using the 3M Petrifilm Plate Reader which provides consistent, fast, automated reading and recording of results of 3M Petrifilm Plates (Aerobic Count, Rapid Aerobic Count, Coliform Count, E. coli /Coliform Count, Enterobacteriaceae Count and Select E. coli Count ) in 4 seconds.

How do you count aerobic bacteria in Petrifilm?

Gel Liquefication and Food Particles Estimated aerobic bacteria count = 160 few species of bacteria liquify the gel in the 3M Petrifilm Aerobic Count Plate. When this occurs, determine the average count in a few unaffected squares and then multiply it by 20 to obtain the estimated count. Do not count red spots within the liquified area.

What is a Petrifilm plate used for?

Versatile: It can be used for air, swab or surface contact environmental sampling The 3M™ Petrifilm™ Aerobic Count Plate is a time-saving, sample-ready plate designed to determine total aerobic bacteria populations. A built-in grid facilitates counting colonies, helping to provide fast, precise and consistent results.

What is the minimum temperature for 3M Petrifilm plates?

Store at temperatures less than or equal to 8°C. 3M Petrifilm Plates provide a significantly more sustainable alternative to traditional agar tests for quantitative microbial indicator testing of food and beverages.