What color is Bacillus megaterium after staining?

While staining Bacillus megaterium for cell wall staining, the Gram-positive bacteria are observed purple and Gram-negative bacteria are observed pink such as rods (bacilli) or round (cocci). Endospore-staining technique is required to stain where the endospores, appear light green oval or spherical structures.

What Gram stain is Bacillus megaterium?

Bacillus megaterium cells stained with Sudan Black B and safranin. Bacillus megaterium is a rod-like, Gram-positive, mainly aerobic spore forming bacterium found in widely diverse habitats. With a cell length of up to 4 µm and a diameter of 1.5 µm, B. megaterium is amongst the biggest known bacteria.

How do you identify Bacillus megaterium?

megaterium are concave, smooth and milk white, while B. mycoides looks white, coarser with fine threads around the colony and the color is milk white. The cell morphology (Figure 3 and Table 1) shows that the cell is rod-shaped, gram positive and sporous.

What is an example of a simple stain microbiology?

Methylene blue is a classic example of a simple stain. This blue stain will color all cells blue, making them stand out against the bright background of the light microscope. Notice below how the background remains generally clear, while the bacterial cells are a deep blue. Methylene blue stains all cells blue.

Is Bacillus megaterium aerobic or anaerobic?

B. megaterium is a Gram-positive, aerobic spore-forming neutralophilic bacterium found in diverse habitats but commonly regarded as a soil bacterium.

What is the morphology of Bacillus megaterium?

Bacillus megaterium is a gram positive, spore producing bacteria. It is a Eubacteria and is found in the soil. It has a rod shape and is one of the largest Eubacteria. Colonies form in chains due to sticky polysaccharides on the cell wall.

Is Bacillus megaterium facultative anaerobic?

Bacillus megaterium is a gram positive, endospore forming, rod shaped bacteria. It is considered aerobic. It is found in soil and considered a saprophyte.

What is simple staining of bacteria?

Simple staining involves directly staining the bacterial cell with a positively charged dye in order to see bacterial detail, in contrast to negative staining where the bacteria remain unstained against a dark background.

What are the most commonly used stains for simple staining procedures?

The Simple Stain Some stains commonly used for simple staining include crystal violet, safranin, and methylene blue.

Why is Bacillus megaterium aerobic?

Bacillus megaterium is a gram positive, endospore forming, rod shaped bacteria. It is considered aerobic. Due to its immense size, about 60 micrometers cubed, B. megaterium has been used to study structure, protein localization and membranes of bacteria since the 1950’s.

Is Bacillus megaterium autotrophic or Heterotroph?

Bacillus subtilis is a motile, Gram-positive, rod-shaped bacteria that occurs as short chains, small clumps, or single cells. It is heterotrophic, which means it cannot make its own food and needs to consume from the environment.

Where does Bacillus megaterium normally live?

Bacillus megaterium is a spore-forming bacterium found in soil, seawater, sediments, rice paddies, dried food, honey, and milk (56).